DMEM (Dulbecco’s Modified Eagle Medium)

Dulbecco’s Modified Eagle Medium (DMEM) is designed to preserve and maintain the growth of a broad spectrum of mammalian cell types.

The DMEM original formulation contains 1000 mg/l of glucose, and is used to culture embryonic mouse cells.

The use of 4500mg/l of glucose in DMEM High Glucose shows an optimal cell growth for some cell lines. DMEM Low Glucose corresponds to the original formulation with 1000 mg/L of glucose.

DMEM was used to culture embryonic mouse cells. DMEM – Ham’s F12 is a mixture (1:1) of DMEM and Ham’s F12.This formulation combines DMEM’s high concentrations of glucose, amino acids, and vitamins with Ham’s F12’s wide variety of components.
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